Mr Weinstein has been accused of dozens of allegations of sexual abuse, including rape, but denies having non-consensual sex.
According to court documents, the lawsuit seeks to “remedy a years-long gender-based hostile work environment.” It targets Harvey Weinstein for allegations of sexual misconduct, as well as the executives and board of The Weinstein Company for failing to protect employees from Weinstein.
Charges were filed in the New York Supreme Court and include new and “extensive” allegations against Weinstein.
New York Attorney General Eric Schneiderman said on Sunday he had filed the suit against the Weinstein Company, Mr Weinstein himself, and his brother Robert.
Meanwhile, the Weinstein Company has held discussions with investors over a possible sale. It is reported that businesswoman Maria Contreras-Sweet led talks to buy the studio for $500m.
Mr Schneiderman said in a statement that “neither perpetrators nor enablers will be unjustly enriched” by any sale of the studio and that the sale “must ensure that victims will be compensated”.
He added that “Every New Yorker has a right to a workplace free of sexual harassment, intimidation, and fear.”